Coding

Part:BBa_K4229035:Design

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-29)


T2 protein from the full wiffleball of the BMC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 34
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 34
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 34
    Illegal AgeI site found at 337
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

The plasmid containing this protein was kindly send to us by the Kerfeld group.


References

H. Kirst and C. A. Kerfeld, “Bacterial microcompartments: Catalysis-enhancing metabolic modules for next generation metabolic and biomedical engineering,” BMC Biol., vol. 17, no. 1, pp. 1–11, 2019, doi: 10.1186/s12915-019-0691-z.